Open Access1,3‐Propanediol production from glycerol by a newly isolated Trichococcus strain
Author(s) -
van Gelder Antonie H.,
Aydin Rozelin,
Alves M. Madalena,
Stams Alfons J. M.
Publication year - 2012
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/j.1751-7915.2011.00318.x
Subject(s) - glycerol , strain (injury) , 1,3 propanediol , formate , propanediol , fermentation , chemistry , bacteria , carbon source , bioreactor , food science , biochemistry , biology , catalysis , organic chemistry , genetics , anatomy
Summary A coccal bacterium (strain ES5) was isolated from methanogenic bioreactor sludge with glycerol as the sole energy and carbon source. Strain ES5 fermented glycerol to 1,3‐propanediol as main product, and lactate, acetate and formate as minor products. The strain was phylogenetically closely related to Trichococcus flocculiformis ; the rRNA gene sequence similarity was 99%. However, strain ES5 does not show the typical growth in chains of T. flocculiformis . Moreover, T. flocculiformis does not ferment glycerol. Strain ES5 used a variety of sugars for growth. With these substrates, lactate, acetate and formate were the main products, while 1,3‐propanediol was not formed. The optimum growth temperature of strain ES5 ranges from 30–37°C, but like several other Trichoccoccus strains, strain ES5 is able to grow at low temperature (< 10°C). Therefore, strain ES5 may be an appropriate catalyst for the biotechnological production of 1,3‐propanediol from glycerol at low ambient temperature.