Open AccessTransgenic expression of glucose dehydrogenase in Azotobacter vinelandii enhances mineral phosphate solubilization and growth of sorghum seedlings
Author(s) -
Sashidhar Burla,
Podile Appa Rao
Publication year - 2009
Publication title -
microbial biotechnology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.287
H-Index - 74
ISSN - 1751-7915
DOI - 10.1111/j.1751-7915.2009.00119.x
Subject(s) - azotobacter vinelandii , gluconic acid , biochemistry , dehydrogenase , azotobacter , escherichia coli , biology , biofertilizer , bacteria , glucose 6 phosphate dehydrogenase , nitrogen fixation , chemistry , microbiology and biotechnology , enzyme , nitrogenase , botany , gene , genetics
Summary The enzyme quinoprotein glucose dehydrogenase (GDH) catalyses the oxidation of glucose to gluconic acid by direct oxidation in the periplasmic space of several Gram‐negative bacteria. Acidification of the external environment with the release of gluconic acid contributes to the solubilization of the inorganic phosphate by biofertilizer strains of the phosphate‐solubilizing bacteria. Glucose dehydrogenase ( gcd ) gene from Escherichia coli , and Azotobacter ‐specific glutamine synthetase ( gln A) and phosphate transport system ( pts ) promoters were isolated using sequence‐specific primers in a PCR‐based approach. Escherichia coli gcd , cloned under the control of gln A and pts promoters, was mobilized into Azotobacter vinelandii AvOP and expressed. Sorghum seeds were bacterized with the transgenic azotobacters and raised in earthen pots in green house. The transgenic azotobacters, expressing E. coli gcd , showed improved biofertilizer potential in terms of mineral phosphate solubilization and plant growth‐promoting activity with a small reduction in nitrogen fixation ability.