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Evaluation of a new rapid anti‐ H b F FITC assay, T rillium Q uik Q uant, for detection and quantitation of foetomaternal haemorrhage
Author(s) -
Fong E. A.,
Finlayson J.,
Robins F.,
Davies J.,
Joseph J.,
Rossi E.,
Grey D. E.
Publication year - 2013
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/j.1751-553x.2012.01463.x
Subject(s) - microbiology and biotechnology , flow cytometry , chemistry , biology
Summary Introduction This study evaluated a new product for flow cytometric foetomaternal haemorrhage ( FMH ) quantitation, T rillium Q uik Q uant anti‐ H b F FITC kit ( T rillium D iagnostics, USA ), in comparison with the M illipore anti‐ H b F FITC method. Methods Blood from 67 antenatal or postpartum R h D ‐negative women together with 108 samples spiked with 0.2%, 0.4%, 0.6%, 2%, 4%, 5%, 6%, 8% and 10% of foetal red cells were analysed by both methods. Results Results for both methods were linear to 10% foetal red cells. Analysis using the W ilcoxon test found that there was no difference between the paired results of T rillium Q uik Q uant and the expected values of the spiked samples ( P = 0.48). In contrast, M illipore anti‐ H b F FITC gave statistically higher results compared with both the expected values and T rillium Q uik Q uant anti‐ H b F FITC ( P = 0.0003 and P < 0.0001, respectively). The B land– A ltman plot for T rillium Q uik Q uant showed a mean difference of only 0.06% below the expected values, whilst M illipore was 0.13% above. The analysis time with T rillium Q uik Q uant was approximately 40 min, requiring significantly less hands‐on time than the M illipore method that required an additional four wash steps. Conclusion Trillium Q uik Q uant is a new precise, accurate and rapid flow cytometric kit method for the quantitation of FMH in both the antenatal and postpartum period.