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Evaluation of CytoDiff ™ on cord blood WBC differential
Author(s) -
Gac F.,
Thibert J. B.,
Berre C.,
Priol J.,
Semana G.,
Fest T.,
Roussel M.
Publication year - 2013
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/j.1751-553x.2012.01460.x
Subject(s) - flow cytometry , cord blood , umbilical cord , immunology , cytometry , cord , haematopoiesis , andrology , medicine , microbiology and biotechnology , biology , stem cell , surgery
Summary Introduction An umbilical cord blood bank was recently opened in our institution as an alternative source of hematopoietic stem cells. Before inclusion of a cord blood in an international register, a WBC with differential is requested, among others. Currently, the reference method is the microscopic manual count, and we sought to evaluate the routine flow cytometric method ( C yto D iff ™ ) as an alternative. Methods A total of 161 cord bloods were analyzed between November 2010 and February 2011. WBC differentials were determined for each sample, by (i) the cell counter (DxH800), (ii) a manual review, and (iii) the flow cytometry using the C yto D iff ™ antibody cocktail. Results Correlation coefficients between flow cytometry and microscopic count were satisfying for neutrophils, lymphocytes, and immature granulocytes and acceptable for eosinophils. On the other hand, we found lower correlation coefficient for basophils and monocytes. Monocytes' correlation was better when comparing flow cytometry with cell counter. Conclusion The flow cytometric approach is suitable to realize cord blood WBC differential and allows for the identification of additional cell subsets.