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Quantification of hemoglobin Constant Spring in heterozygote and homozygote by a capillary electrophoresis method
Author(s) -
PORNPRASERT S.,
PANYASAI S.,
WANEESORN J.,
KONGTHAI K.,
SINGBOOTTRA P.
Publication year - 2012
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/j.1751-553x.2011.01371.x
Subject(s) - heterozygote advantage , mean corpuscular volume , hemoglobin , capillary electrophoresis , hemoglobin c , hemoglobin variants , chemistry , medicine , microbiology and biotechnology , chromatography , biology , gene , genotype , biochemistry
Summary Introduction: Capillary electrophoresis (CE) is a high‐resolution method for detection of hemoglobin Constant Spring (Hb CS). Methods: The levels of Hb CS quantified by CE were compared among three groups of samples including heterozygote and homozygote of Hb CS as well as Hb H‐CS disease classified by DNA molecular diagnosis. The mean corpuscular volume (MCV) of red blood cells was also analyzed among these three groups. Results: Mean ± SD of Hb CS level of the homozygote was not significantly different from that of the Hb H‐CS disease (1.9 ± 1.8 vs . 2.8 ± 1.3, P = 0.13), but it was significantly higher than that of the heterozygote (1.9 ± 1.8 vs . 0.4 ± 0.2, P = 0.007). The MCV <70 fL was found in Hb H‐CS disease only. Conclusion: CE is the preferable method for screening of heterozygote and homozygote of Hb CS. Moreover, in conjunction with a lower MCV (<70 fL), this approach provided a high resolution to identify Hb H‐CS disease.