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Low hemoglobin density potential marker of iron availability
Author(s) -
URRECHAGA E.,
UNCETA M.,
BORQUE L.,
ESCANERO J. F.
Publication year - 2012
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/j.1751-553x.2011.01355.x
Subject(s) - medicine , receiver operating characteristic , hemoglobin , gastroenterology , anemia , iron deficiency anemia , soluble transferrin receptor , iron deficiency , anemia of chronic disease , iron status
Summary Introduction: Low hemoglobin density (LHD%) is a new parameter provided by Beckman‐Coulter derived from the mean cell hemoglobin concentration, using the mathematical sigmoid transformationThis study investigated the reliability of LHD% for the assessment of iron status in the presence of inflammation. Methods: Healthy subjects ( n = 90) and patients with iron deficiency (IDA, n = 110), chronic kidney disease (CKD, n = 65) and anemia of chronic disease (ACD, n = 85; 24 were iron deficient and 61 were iron sufficient) were analyzed on a LH 780 analyzer (Beckman Coulter Inc., Miami, FL, USA). Independent samples U test and receiver operating characteristic (ROC) curve analysis were applied. To determine the concordance between LHD% and soluble transferrin receptor (sTrR) Cohen’s κ index was calculated. Results: LHD % values showed no statistical difference in patients with IDA and patients with ACD accompanied with IDA ( P = 0.6427); LHD% values in these patients were significantly different ( P < 0.0001) compared with the iron‐sufficient patients with ACD. ROC analysis for LHD% in the detection of iron deficiency showed the following: area under curve 0.903; cut off 5.5%, sensitivity 88.6%, specificity 76.9%; κ index, 0.65. Conclusion: LHD% is a reliable parameter for the detection of iron deficiency in patients with anemia in the presence of inflammation.