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An allelic typing method for 2DS4 variant used in study of haplotypes of killer cell immunoglobulin‐like receptor gene
Author(s) -
BAO X.,
HOU L.,
SUN A.,
CHEN M.,
CHEN Z.,
HE J.
Publication year - 2010
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/j.1751-553x.2010.01234.x
Subject(s) - haplotype , allele , hematopoietic stem cell transplantation , exon , typing , gene , biology , transplantation , medicine , genetics , immunology , stem cell
Summary The KIR2DS4 variants differ in exon 5 and play a role in hematopoietic stem cells transplantation (HSCT). A sequence‐based testing (SBT) and TOPO TA cloning system identifying and distinguishing alleles of the KIR2DS4 gene was established and applied to a total of 150 Chinese‐Han individuals: 75 patients received T‐cell‐depleted HSCT and their unrelated donors. The majority (139) of the 150 samples (92.7%) were positive for KIR2DS4. Four of the nine known KIR2DS4 alleles, KIR2DS4 *00101, *003,*004, and *007, were identified. In the haplotype A/A group, a higher risk of acute graft‐versus‐host disease (aGVHD) was seen when the donor carried two full‐length KIR2DS4 alleles (RR 9.0 [95% CI 1.2–66.9], P = 0.010). Our findings suggested that the expression of full‐length 2DS4 (*001) in A/A group may contribute to a worse clinical outcome after URD‐HSCT. These data would be beneficial for the selection of suitable donors.