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Apoptosis inducing and differentiation enhancement effect of oridonin on the all‐trans‐retinoic acid‐sensitive and ‐resistant acute promyelocytic leukemia cells
Author(s) -
GAO F.,
TANG Q.,
YANG P.,
FANG Y.,
LI W.,
WU Y.
Publication year - 2010
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/j.1751-553x.2009.01147.x
Subject(s) - acute promyelocytic leukemia , retinoic acid , apoptosis , leukemia , reactive oxygen species , differentiation therapy , cancer research , chemistry , cellular differentiation , catabolism , biology , microbiology and biotechnology , biochemistry , immunology , gene , metabolism
Summary We investigated the effects of oridonin (Ori), a diterpenoid isolated from Rabdosia rubescens , on apoptosis and differentiation of all‐trans‐retinoic acid (ATRA)‐sensitive (NB4) and ATRA‐resistant (NB4‐R1) cells. The results showed that reactive oxygen species initiates Ori‐induced apoptosis. In addition, we found that neither Ori nor ATRA (10 n m ) alone induced marked cell differentiation, while co‐treatment of these two compounds can induce differentiation of NB4 and NB4‐R1 cells which was accompanied by increased RARα, C/EBPε or C/EBPβ. This is the first report to show that RARα could be accumulated by Ori which may be useful as a probe to investigate the mechanism of RARα catabolism. These results suggest that Ori is a potential candidate for acute promyelocytic leukemia cancer therapy.