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In vitro studies with ‘acatalasemic‐like’ erythrocytes and hydrogen peroxide: attention to the formation of lysis resistant erythrocytes
Author(s) -
NASCIMENTO H.,
BELO L.,
FERNANDES J.,
ROCHA S.,
QUINTANILHA A.,
SANTOSSILVA A.
Publication year - 2010
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/j.1751-553x.2008.01129.x
Subject(s) - hemolysis , hydrogen peroxide , lysis , catalase , sodium azide , chemistry , in vitro , red blood cell , biochemistry , biology , immunology , enzyme
Summary Hemolysis assays, using hydrogen peroxide as an oxidant injury agent and sodium azide as a catalase inhibitor, were performed to evaluate how assay conditions affect the development of lysis resistant erythrocytes. The concentration of hydrogen peroxide should be carefully selected for use in hemolysis assays, as it was found to affect the number of water resistant erythrocytes.