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A rapid flow cytometric technique for the detection of platelet‐monocyte complexes, activated platelets and platelet‐derived microparticles
Author(s) -
PEARSON LAURA,
THOM JIM,
ADAMS MURRAY,
OOSTRYCK ROBERT,
KRUEGER ROM,
YONG GERALD,
BAKER ROSS
Publication year - 2009
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/j.1751-553x.2008.01059.x
Subject(s) - platelet , platelet activation , monocyte , p selectin , chemistry , flow cytometry , medicine , conventional pci , immunology , endocrinology , myocardial infarction
Summary Platelet activation occurs in a variety of clinical situations in which it directly contributes to the pathology. This study reports a simple flow cytometric assay for platelet activation which measures platelet‐derived microparticles, activated platelets and platelet–monocyte complexes. Pre‐ and post analytical conditions were investigated and optimized and a normal range established on 20 healthy controls. Twenty patients pre‐ and post percutaneous coronary intervention (PCI) were tested with the technique. Soluble activation markers sCD40 ligand and sP‐selectin and plasma phospholipid levels were measured in both groups. There was a significant increase in activated platelets and platelet–monocyte complexes between normal and pre‐PCI ( P = 0.005 and 0.0275, respectively) suggesting an activated state. There was a significant fall in activated platelets post‐PCI ( P = 0.0027) which was mirrored by a fall in soluble CD40 ligand, soluble P‐selectin and plasma phospholipid levels ( P = 0.0066, <0.0001 and 0.0032, respectively) consistent with antiplatelet therapy administered during the process. This is a reliable and rapid method for the assessment of ex vivo platelet activation which may be an aid in diagnosis and help guide therapy for patients with thrombotic disease.