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Experience with sodium fluorescein flow cytometry in the determination of red cell volume
Author(s) -
SLIGHT R. D.,
FERGUSON R.,
STIRLING D.,
McCLELLAND D. B. L.,
MANKAD P. S.
Publication year - 2009
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/j.1751-553x.2008.01029.x
Subject(s) - flow cytometry , fluorescein , repeatability , sodium fluorescein , red cell , volume (thermodynamics) , population , fluorescence , red blood cell , sodium , chemistry , biophysics , chromatography , biology , microbiology and biotechnology , medicine , optics , biochemistry , physics , environmental health , organic chemistry , quantum mechanics
Summary Sodium fluorescein flow cytometry for the calculation of red cell volume is an exciting proposition in that the repeatability of the technique in a short time frame should allow for applications such as the measurement of surgical red cell volume loss. Our results found that the rapid decay in fluorescence negated the usefulness of this technique as currently described. However, further investigation into the behaviour of the sodium fluorescein labelled red blood cells may allow for the mathematical correction of the fluorescent red cell population prior to red cell volume calculation.