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Conjugation of methotrexate to immunoglobulins kills macrophages by F c receptor mediated uptake?
Author(s) -
WANG X.,
YAO C.,
JIANG Z.
Publication year - 2008
Publication title -
international journal of laboratory hematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.705
H-Index - 55
eISSN - 1751-553X
pISSN - 1751-5521
DOI - 10.1111/j.1751-553x.2007.00936.x
Subject(s) - conjugate , cytotoxicity , trypan blue , methotrexate , in vitro , flow cytometry , chemistry , antibody , hela , pharmacology , receptor , microbiology and biotechnology , immunology , biochemistry , biology , mathematical analysis , mathematics
Summary The aim of this study was to conjugate methotrexate (MTX) with intravenous immunoglobulin (IVIG) and investigate whether the conjugate produce selective cytotoxicity on macrophages to provide a new strategy for the management of idiopathic thrombocytopenic purpura. MTX was bound to IVIG via human serum albumin as an intermediary. The binding activity of the F c fragment of the conjugate was assayed by flow cytometry. The selective cytotoxicity of the conjugate was determined by trypan blue exclusion. After conjugating, the binding activity of the conjugate to F c receptors did not diminish when compared with IVIG. In vitro , the conjugate showed significantly higher cytotoxicity to macrophages than Hela cells. The conjugate of IVIG and MTX showed potent and selective cytotoxicity to macrophages in vitro .