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Hematopoietic stem cell mobilization into the peripheral circulation in patients with chronic liver diseases
Author(s) -
Ghanem Lobna Y,
Nosseir Mona MF,
Lotfi Abeya A,
Mohamed Ayat S,
Ibrahim Refaat A,
Hassanein Moutaz H,
Mansour Eman,
Makhlouf Manal M,
Fouad Yasser M,
ElKhayat Hisham R
Publication year - 2012
Publication title -
journal of digestive diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 51
eISSN - 1751-2980
pISSN - 1751-2972
DOI - 10.1111/j.1751-2980.2012.00634.x
Subject(s) - homing (biology) , bone marrow , progenitor cell , haematopoiesis , medicine , stem cell , liver injury , hematopoietic stem cell transplantation , pathology , mobilization , chronic liver disease , transplantation , biology , cirrhosis , microbiology and biotechnology , ecology , history , archaeology
Objective The present study was aimed to investigate and compare the kinetics of bone marrow‐derived hematopoietic stem cells ( BMHSC ) migration in the peripheral blood and liver in response to liver injury in patients with chronic liver disease ( CLD ). Methods In all, 45 CLD patients staged with C hild– P ugh A, B and C and 15 healthy participants were evaluated for the concentration of circulating BMHSC by a flow cytometric analysis of CD 133 + / CD 34 + cells. In addition, homing BMHSC and hepatic progenitors were assessed by the immunohistochemical detection of CD 133 + and OV 6 + cells in liver biopsy specimens from C hild– P ugh A and B patients. Results No significant difference in the percentage of circulating CD 133 + / CD 34 + cells was observed among all groups of patients. In liver tissues, OV 6 + cells increased significantly in C hild– P ugh B cases ( P < 0.05), while CD 133 + cells were distributed sparsely in the periportal region in C hild– P ugh A and B patients. OV 6 + cells were significantly correlated with CD 34 + cells but not with CD 133 + cells in C hild– P ugh A and B patients ( P < 0.01 and P < 0.05, respectively). Conclusions Various degrees of severity in CLD neither evoked the mobilization of BMHSC into the circulation nor triggered their homing into liver tissue, thus excluding extrahepatic stem cell‐mediated repair. The recovery process seems to be dependent on proliferating endogenous liver progenitors ( OV 6 + cells).