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Fractalkine as a marker for assessment of severe acute pancreatitis
Author(s) -
HUANG Li Ya,
CHEN Ping,
XU Ling Xiao,
ZHOU Yu Fen,
LI Wei Guang,
YUAN Yao Zong
Publication year - 2012
Publication title -
journal of digestive diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 51
eISSN - 1751-2980
pISSN - 1751-2972
DOI - 10.1111/j.1751-2980.2012.00580.x
Subject(s) - immunohistochemistry , medicine , western blot , pancreas , pancreatitis , reverse transcription polymerase chain reaction , kidney , messenger rna , lung , real time polymerase chain reaction , reverse transcriptase , pathology , acute pancreatitis , polymerase chain reaction , biology , gene , biochemistry
OBJECTIVE: The aim of this study was to study the role of fractalkine (FKN) in the development of severe acute pancreatitis (SAP) in animal model. METHODS: Serum FKN levels in rat model (control, SAP6h, 16h, 24h and 48h) were determined by enzyme‐linked immunosorbent assay. FKN mRNA and protein levels in the pancreas tissue were measured by reverse transcription polymerase chain reaction (RT‐PCR), Western blot and immunohistochemistry. RESULTS: Serum FKN level in the SAP rat increased significantly ( P < 0.05 compared with the control group). FKN mRNA and protein levels in pancreas and lung increased significantly and reached the peak at 16 h after the induction of SAP, while those in kidney reached the peak at 48 h. Immunohistochemistry showed the overexpression of FKN in pancreas, lung and kidney tissue. CONCLUSION: FKN involves in the progression of SAP and might be a valuable marker for the assessment of SAP.