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Effect of enteral nutrition on dextran sulfate sodium‐induced colitis in rats
Author(s) -
WANG Fang,
ZHAO Hai Ying,
ZHANG Shu Tian,
GONG You Zhe,
ZHANG Hai Fang,
ZHANG Chuan
Publication year - 2011
Publication title -
journal of digestive diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 51
eISSN - 1751-2980
pISSN - 1751-2972
DOI - 10.1111/j.1751-2980.2011.00518.x
Subject(s) - glutamine , medicine , colitis , enteral administration , gastroenterology , lactobacillus , parenteral nutrition , enema , food science , biochemistry , chemistry , amino acid , fermentation
OBJECTIVE: To study the effect of enteral nutrition (EN) on dextran sulfate sodium (DSS)‐induced colitis in rats. METHODS: Eighty‐four Sprague–Dawley rats were divided into 7 groups (12 rats in each group). The blank control group was given ordinary laboratory feed and drinking water. The experimental groups received 5% DSS as drinking water for 7 days. Of the experimental groups, the model control group received ordinary laboratory feed, protein based enteral nutrition (PEN) was fed in the PEN group, while other groups received ordinary laboratory feed plus 5‐aminosalicylic acid (5‐ASA), methyl‐prednisolone, Lactobacillus or glutamine, respectively. On the 8th day, all the rats were sacrificed. Inflammatory scores were assessed from colonic mucosa. Blood culture from inferior vena cava, fecal culture and secretary immunoglobulin‐A (S‐IgA) levels from colonic contents were determined. RESULTS: Colon inflammatory scores of Lactobacillus , PEN, glutamine and drug‐treated groups were lower than that of the model control group ( P < 0.01). The ratios of bacteria translocation in the EN (PEN, Lactobacillus and glutamine) groups were lower than that in the model control group ( P < 0.0083). Fecal Lactobacilli in the Lactobacillus and glutamine groups were higher than that in the model control group ( P < 0.05). S‐IgA levels in colonic contents of the PEN and 5‐ASA group were lower than that in the model control group ( P < 0.05). CONCLUSIONS: EN is an effective therapy for treating DDS‐induced colitis. EN could alleviate damage, promote the repair of colonic epithelial cells and inhibit bacterial translocation. Lactobacillus and glutamine could also increase the Lactobacilli in colon.