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Preliminary experimental research on the mechanism of liver bile secretion stimulated by peppermint oil
Author(s) -
ZONG Lei,
QU Ying,
LUO Di Xiang,
ZHU Zhi Yong,
ZHANG Sheng,
SU Zhi,
SHAN Jian Cheng,
GAO Xiao Ping,
LU Lun Gen
Publication year - 2011
Publication title -
journal of digestive diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 51
eISSN - 1751-2980
pISSN - 1751-2972
DOI - 10.1111/j.1751-2980.2011.00513.x
Subject(s) - choleretic , cholesterol 7 alpha hydroxylase , farnesoid x receptor , bile acid , medicine , secretion , cholesterol , endocrinology , downregulation and upregulation , messenger rna , chemistry , biochemistry , transcription factor , gene , nuclear receptor
OBJECTIVE: To investigate the choleretic effect and molecular mechanisms of action of peppermint oil (PO), the main component of Danshu capsules (Sichuan Jishengtang Pharmaceutical Co., Ltd., Pengzhou, Sichuan Province, China). METHODS: Bile secretion was measured by biliary drainage in rats. Total bile acids, total cholesterol and bilirubin in bile were determined. Cholesterol 7α‐hydroxylase (CYP7A1), and farnesoid X receptor (FXR) messenger ribonucleic acid (mRNA) levels were assessed in HepG2 cells (a human hepatocellular carcinoma cell line) by reverse transcription polymerase chain reaction (RT‐PCR). RESULTS: PO significantly promoted bile and bile acid secretion in rats. It also increased bile acid efflux and decreased cholesterol levels ( P < 0.01) in bile. In HepG2 cells the mRNA levels of CYP7A1 and FXR were significantly upregulated after treatment with PO. CONCLUSIONS: PO stimulates bile fluid secretion and thus has a choleretic effect. PO might play a role in upregulating CYP7A1 and FXR mRNA levels, suggesting that the molecular mechanisms are related to gene expression involved in bile acid synthesis.