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Involvement of VDAC1 and Bcl‐2 family of proteins in VacA‐induced cytochrome c release and apoptosis of gastric epithelial carcinoma cells
Author(s) -
LAN Chun Hui,
SHENG Jian Qiu,
FANG Dian Chun,
MENG Qing Zhuang,
FAN Li Lin,
HUANG Zeng Rong
Publication year - 2010
Publication title -
journal of digestive diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.684
H-Index - 51
eISSN - 1751-2980
pISSN - 1751-2972
DOI - 10.1111/j.1751-2980.2009.00412.x
Subject(s) - vdac1 , cytochrome c , apoptosis , voltage dependent anion channel , transfection , microbiology and biotechnology , bcl 2 family , bcl 2 associated x protein , downregulation and upregulation , biology , blot , chemistry , caspase 3 , programmed cell death , biochemistry , bacterial outer membrane , gene , escherichia coli
OBJECTIVE: It is known that the vacuolating cytotoxin (VacA) could induce apoptosis. However, the mechanism remained to be elucidated. The aim of this study is to investigate the role of Bcl family of proteins (Bcl‐2 and Bax) and the mitochondrial voltage‐dependent anion channel (VDAC) in VacA‐induced apoptosis of AGS cells. METHODS: Plasmid pGBKT7‐VacA p58 was constructed and transfected into the AGS cells. RT‐PCR and Western blotting were used to determine the expressions of cytochrome c, caspase‐3, Bax, Bcl‐2 and VDAC1 mRNA and proteins. RESULTS: VacA p58 can induce cytochrome c release and activate caspase‐3 in AGS cells. It up‐regulated the expressions of Bax and VDAC1 mRNA and proteins, and decreased the expression of Bcl‐2 in AGS cells. CONCLUSION: VacA p58 induces apoptosis in AGS cells. This apoptotic process is associated with the up‐regulation of Bax/VDAC1 and downregulation of Bcl‐2. These findings suggest that the release of cytochrome c by VacA p58 is mainly through VDAC‐dependent and Bcl‐2 family‐dependent pathways.