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Low‐level Laser Therapy Ameliorates CC l 4 ‐induced Liver Cirrhosis in Rats
Author(s) -
OliveiraJunior Manoel Carneiro,
Monteiro Aldaíza Salomão,
LealJunior Ernesto César Pinto,
Munin Egberto,
Osório Rodrigo Aléxis Lazo,
Ribeiro Wellington,
Vieira Rodolfo Paula
Publication year - 2012
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.2012.01211.x
Subject(s) - alkaline phosphatase , lactate dehydrogenase , cirrhosis , carbon tetrachloride , medicine , liver function , endocrinology , inflammation , chemistry , ccl4 , globulin , hepatic stellate cell , biochemistry , enzyme , organic chemistry
This study investigated the effects of low‐level laser therapy ( LLLT ) in the liver function, structure and inflammation in a experimental model of carbon tetrachloride ( CCl 4 )‐induced liver cirrhosis. Wistar rats were divided into Control, LLLT , CCl 4 and CCl 4 + LLLT groups. CCl 4 groups received CCl 4 (0.4 g kg −1 ; i.p.), three times a week, for 12 weeks. A 830 nm LLLT was performed with a continuous wave, 35 mW, 2.5 J cm −2 per point, applied to four points of the liver (right and left upper and lower extremities, in the four lobes of the liver) for 2 weeks. Liver structure and inflammation (cirrhotic areas, collagen deposition, inflammation, density of K upffer and hepatic stellate cells) and function (aspartate aminotransferase, alkaline phosphatase, gamma glutamyltransferase, lactate dehydrogenase, total proteins and globulins) were evaluated. LLLT significantly reduced CCl 4 ‐increased aspartate aminotransferase ( P < 0.001), alkaline phosphatase ( P < 0.001), gamma‐glutamyl transferase ( P < 0.001) and lactate dehydrogenase ( P < 0.01) activity, as well as total proteins ( P < 0.05) and globulins ( P < 0.01). LLLT also reduced the number of cirrhotic areas, the collagen accumulation and the hepatic inflammatory infiltrate. Of note, LLLT reduced CCl 4 ‐increased number of K upffer cells ( P < 0.05) and hepatic stellate cells ( P < 0.05). We conclude that LLLT presents beneficial effects on liver function and structure in an experimental model of CCl 4 ‐induced cirrhosis.