Premium
Influence of Helium‐Neon Laser Irradiation on Seed Germination In Vitro and Physico‐Biochemical Characters in Seedlings of Brinjal ( Solanum melongena L.) var. Mattu Gulla
Author(s) -
Muthusamy Annamalai,
Kudwa Prathibha P.,
Prabhu Vijendra,
Mahato Krishna K.,
Babu Vidhu Sankar,
Rao Mattu Radhakrishna,
Gopinath Puthiya Mandyat,
Satyamoorthy Kapaettu
Publication year - 2012
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.2012.01162.x
Subject(s) - germination , solanum , melongena , horticulture , irradiation , shoot , carotenoid , biology , chlorophyll , botany , chemistry , physics , nuclear physics
In the present study, the seeds of brinjal ( Solanum melongena L.) var. Mattu Gulla were irradiated with single exposure of He–Ne laser at different doses of 5–40 J cm −2 and germinated aseptically. Thirty day old seedlings were harvested and the germination, growth, physiological and biochemical parameters were estimated and compared with un‐irradiated control seedlings. A significant enhancement in growth characters were noted with respect to length, fresh and dry weight of shoots and roots. In addition, chlorophyll ( a and b ), carotenoid content, anthocyanin and amylases (α and β) activities were found to be altered. Significant alterations in percentage of seed germination ( P < 0.001) and time to 50% germination ( P < 0.001) were observed in the irradiated seeds compared with the un‐irradiated controls. In conclusion, the results of the present study demonstrated that low dose (5–30 J cm −2 ) of He–Ne laser irradiation enhanced the germination process and altered growth, by positively influencing physiological and biochemical parameters of the brinjal seedlings compared with un‐irradiated control under in vitro conditions.