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Monitoring Microcirculation Changes in Port Wine Stains During Vascular Targeted Photodynamic Therapy by Laser Speckle Imaging
Author(s) -
Qiu Haixia,
Zhou Yang,
Gu Ying,
Ang Qing,
Zhao ShiYong,
Wang Ying,
Zeng Jing,
Huang Naiyan
Publication year - 2012
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.2012.01153.x
Subject(s) - photodynamic therapy , port wine , perfusion , microcirculation , medicine , nuclear medicine , perfusion scanning , pathology , radiology , surgery , chemistry , organic chemistry
This study was conducted to test laser speckle perfusion imaging (LSPI) for imaging microcirculation and monitoring microcirculatory changes of port wine stains (PWS) during vascular targeted photodynamic therapy (V‐PDT). Before and 5 min after V‐PDT, PWS lesions and the corresponding contralateral healthy skins of 24 PWS patients were scanned, whereas seven PWS patients were scanned throughout V‐PDT. V‐PDT was conducted immediately after intravenous injection of photocarcinorin (4–5 mg kg −1 ). A 532 nm laser was used for irradiation (power density: 80–100 mW cm −2 , exposure time: 20–50 min). Before V‐PDT, all 24 PWS patients demonstrated a significant difference in perfusion between the PWS lesion and the contralateral healthy control skin (1132 ± 724 and 619 ± 478 PU, respectively, P < 0.01). Five minutes after V‐PDT, the mean perfusion value of the 24 PWS lesions was 1246 ± 754 PU. There was no significant difference compared to the perfusion before V‐PDT ( P > 0.05). During V‐PDT, the perfusion of seven PWS patients increased rapidly after initiation of V‐PDT, reached a maximum within 10 min, lasted for several minutes, and slowly returned to a relatively lower level at the end of V‐PDT. On the basis of these results, LSPI is capable of imaging PWS microvasculature and monitoring microvascular reactivity to V‐PDT.