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Phloroglucinol Attenuates Ultraviolet B Radiation‐Induced Matrix Metalloproteinase‐1 Production in Human Keratinocytes via Inhibitory Actions against Mitogen‐Activated Protein Kinases and Activator Protein‐1
Author(s) -
Piao Mei Jing,
Zhang Rui,
Lee Nam Ho,
Hyun Jin Won
Publication year - 2012
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.2012.01074.x
Subject(s) - hacat , phloroglucinol , chemistry , kinase , photoaging , matrix metalloproteinase , activator (genetics) , microbiology and biotechnology , protein kinase a , reactive oxygen species , oxidative stress , phosphorylation , p38 mitogen activated protein kinases , downregulation and upregulation , human skin , biochemistry , biology , receptor , gene , genetics , in vitro , organic chemistry
Excessive amounts of reactive oxygen species (ROS) induced by ultraviolet (UV) radiation cause skin aging via basement membrane/extracellular matrix degradation resulting from the action of matrix metalloproteinases (MMPs). Recently, phloroglucinol (1,3,5‐trihydroxybenzene) was demonstrated to attenuate the cell damage induced by oxidative stress by quenching ROS and stimulating antioxidant systems. In the current study, the effect of phloroglucinol on UVB‐induced photoaging was investigated in human HaCaT keratinocytes. Phloroglucinol significantly inhibited the UVB‐induced (1) upregulation of MMP‐1 mRNA, protein and activity; (2) augmentation of intracellular Ca 2+ levels; (3) phosphorylation of mitogen‐activated protein kinases (MAPKs); (4) expression of c‐Fos and phospho c‐Jun; and (5) enhancement of activator protein‐1 (AP‐1) binding to the MMP‐1 promoter. In addition, the knockdown of MAPKs significantly inhibited UVB‐induced MMP‐1 expression. The results of this study suggest that phloroglucinol may be useful as a photoprotective compound for the skin.