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In Vitro Fungicidal Photodynamic Effect of Hypericin on Candida Species †
Author(s) -
Rezusta Antonio,
LópezChicón Patricia,
PazCristobal Manuel P.,
AlemanyRibes Mireia,
RoyoDíez David,
Agut Montserrat,
Semino Carlos,
ll Santi,
Revillo Mª Jose,
Aspiroz Carmen,
Gilaberte Yolanda
Publication year - 2011
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.2011.01053.x
Subject(s) - hypericin , photodynamic therapy , candida albicans , photosensitizer , candida parapsilosis , fungicide , chemistry , corpus albicans , candida krusei , in vitro , hacat , microbiology and biotechnology , biology , pharmacology , photochemistry , botany , biochemistry , organic chemistry
Hypericin is a natural photosensitizer considered for the new generation of photodynamic therapy (PDT) drugs. The aim of this study was to evaluate the in vitro fungicidal effect of hypericin PDT on various Candida spp., assessing its photocytotoxicity to keratinocytes (HaCaT) and dermal fibroblasts (hNDF) to determine possible side effects. A 3 log fungicidal effect was observed at 0.5 McFarland for two Candida albicans strains, Candida parapsilosis and Candida krusei with hypericin concentrations of 0.625, 1.25, 2.5 and 40 μ m , respectively, at a fluence of 18 J cm −2 (LED lamp emitting at 602 ± 10 nm). To obtain a 6 log reduction, significantly higher hypericin concentrations and light doses were needed ( C. albicans 5 μ m , C. parapsilosis 320 μ M and C. krusei 320 μ M ; light dose 37 J cm −2 ). Keratinocytes and fibroblasts can be preserved by keeping the hypericin concentration below 1 μ m and the light dose below 37 J cm −2 . C. albicans appears to be suitable for treatment with hypericin PDT without significant damage to cutaneous cells.