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Photodynamic Action of Rose Bengal Silica Nanoparticle Complex on Breast and Oral Cancer Cell Lines
Author(s) -
Uppal Abha,
Jain Beena,
Gupta Pradeep Kumar,
Das Kaustuv
Publication year - 2011
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.2011.00967.x
Subject(s) - rose bengal , phototoxicity , singlet oxygen , photosensitizer , nanoparticle , conjugated system , zeta potential , photodynamic therapy , chemistry , covalent bond , dynamic light scattering , biophysics , photochemistry , combinatorial chemistry , nanotechnology , materials science , organic chemistry , biochemistry , in vitro , oxygen , polymer , biology
Rose Bengal, an anionic photosensitizer was conjugated to organically modified silica nanoparticles having 3‐amino propyl groups by electrostatic or covalent interaction. The drug‐nanoparticle complexes were characterized by FTIR, light scattering and zeta potential measurements. Significant changes were observed in the spectroscopic properties of the drug when it is conjugated with nanoparticles. The toxicity of the free drug and drug‐nanoparticle complex was studied against oral (4451) and breast (MCF‐7) cancer cell lines. Both complexes with nanoparticles were more phototoxic than free Rose Bengal, with the covalent complex being the more effective. Studies carried out on cellular uptake, photostability and singlet oxygen generation suggest that enhanced phototoxicity is primarily due to the enhanced uptake of the drug‐nanoparticle complex.