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Photobleaching Kinetics of Verteporfin and Lemuteporfin in Cells and Optically Trapped Multilamellar Vesicles Using Two‐photon Excitation
Author(s) -
Tekrony Amy D.,
Kelly Nicole M.,
Fage B. Alexander,
Cramb David T.
Publication year - 2011
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.2011.00933.x
Subject(s) - photobleaching , verteporfin , excitation , kinetics , two photon excitation microscopy , vesicle , chemistry , fluorescence recovery after photobleaching , biophysics , physics , optics , fluorescence , biology , biochemistry , quantum mechanics , choroidal neovascularization , membrane , visual acuity
Verteporfin and Lemuteporfin are compared to examine the effect of their functional groups and therefore the localization in two‐photon excitation (TPE) photodynamic therapy (PDT). We used singlet oxygen‐related photobleaching of the sensitizers to assess TPE‐induced singlet oxygen generation in multilamellar vesicles (MLVs) and U343 glioma cells under a variety of conditions. It was found that Lemuteporfin photobleached at a faster rate than Verteporfin in the majority of environments. Also, Verteporfin and Lemuteporfin exhibited different behaviors when in hypoxic environments relative to those in oxygenated MLVs. These differences are attributed to the sensitizer location in the membrane and their relative mobilities throughout membranes and cells.