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Photodynamic Effects of Pterin on HeLa Cells
Author(s) -
Paula Denofrio M.,
Lorente Carolina,
Breitenbach Thomas,
Hatz Sonja,
Cabrerizo Franco M.,
Thomas Andrés H.,
Ogilby Peter R.
Publication year - 2011
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.2011.00922.x
Subject(s) - trypan blue , hela , viability assay , programmed cell death , chemistry , rhodamine 123 , cell , mitochondrion , rhodamine , pterin , fluorescence , apoptosis , biophysics , microbiology and biotechnology , biology , biochemistry , cofactor , antibiotics , physics , quantum mechanics , multiple drug resistance , enzyme
Pterins, heterocyclic compounds widespread in biological systems, participate in relevant biological processes and are able to act as photosensitizers. In the present study, we ascertained that 2‐aminopteridin‐4(3 H )‐one, abbreviated as Ptr, is readily incorporated into and/or onto cervical cancer cells (HeLa) and that these cells die upon UV‐A irradiation of Ptr. Cell death was assessed using two tests: (1) the Rhodamine 123 fluorescence assay for mitochondrial viability and (2) the Trypan Blue assay for membrane integrity. The data suggest that, for Ptr‐dependent photoinitiated cell death, events related to mitochondrial failure precede those associated with the failure of the cell membrane.