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Detection of Nucleic Acid Lesions During Photochemical Inactivation of RNA Viruses by Treatment with Methylene Blue and Light Using Real‐time PCR
Author(s) -
Zhang Bo,
Zheng Lan,
Huang Yuwen,
Mo Qin,
Wang Xun,
Qian Kaicheng
Publication year - 2011
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.2010.00870.x
Subject(s) - infectivity , nucleic acid , rna , sindbis virus , methylene blue , biology , virology , microbiology and biotechnology , virus , bacteriophage , chemistry , biochemistry , gene , escherichia coli , photocatalysis , catalysis
The mechanism of bacteriophage photoinactivation by methylene blue and light (MB+L) involves genomic RNA damage. In this study, two RNA viruses, Sindbis virus (SINV) and hepatitis C virus were treated by MB+L and their nucleic acids were amplified to show that RNA lesions occurred during inactivation. During MB+L inactivation, the viral load of both viruses was significantly reduced as MB+L exposure increased. The nucleic acid amplification of treated viral RNA was inhibited in a time‐dependent manner and the percentage inhibition of amplification reached about 99% after 30 min of treatment. Furthermore, as compared to SINV viral infectivity detected by quantification of the 50% tissue culture infective dose (TCID 50 ), the inhibition of SINV RNA amplification strongly correlated with a decrease in in vitro infectivity ( R 2 > 0.94), suggesting that RNA serves as the main target during MB+L inactivation.