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Peculiar Properties of Photoinduced Hydroxylaminolysis in Different Bacteriorhodopsin‐based Media Using O‐Substituted Hydroxylamines
Author(s) -
Dyukova Tatyana V.,
Druzhko Anna B.
Publication year - 2010
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.2010.00817.x
Subject(s) - hydroxylamine , hydroxylamine hydrochloride , bacteriorhodopsin , chemistry , hydrochloride , photosensitivity , gelatin , photochemistry , molecule , nuclear chemistry , organic chemistry , materials science , biochemistry , optoelectronics , membrane
The process of photoinduced hydroxylaminolysis has been re‐examined in different bacteriorhodopsin (BR)‐based media using O‐substituted hydroxylamines, in particular, O‐(4‐nitrobenzyl) hydroxylamine hydrochloride (NBHA), O‐(2,3,4,5,6‐pentafluorobenzyl) hydroxylamine hydrochloride (FBHA) and O‐( t ‐butyl) hydroxylamine hydrochloride (BHA). Both wild type (WT) and D96N BR‐based gelatine films and gels were studied. The expected increase in the bleaching rate of BR in gelatin films by using O‐substituted hydroxylamines in place of HA was not achieved. On the other hand, it was shown that in gels HA derivatives NBHA and FBHA (as against HA itself) do provide about three‐ to four‐fold higher bleaching rate. By contrast to that in films, D96N BR in gels demonstrates more effective bleaching as compared to WT BR. The plausible interpretation for the results is discussed in frames of reduced mobilities of large‐sized molecules of O‐substituted hydroxylamines in dehydrated media. FBHA‐ or NBHA‐modified gels possess higher photosensitivity both with D96N and WT BR (as compared with that for HA‐modified gels) and offer a potentiality for application as an irreversible‐recording medium. As anticipated, it is specifically D96N BR gel modified with FBHA that may present a promising medium suitable for write‐once recording thus extending the range of recording materials in the optical processing field.