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19 F‐MAS NMR on Proteorhodopsin: Enhanced Protocol for Site‐Specific Labeling for General Application to Membrane Proteins †
Author(s) -
Hellmich Ute A.,
Pfleger Nicole,
Glaubitz Clemens
Publication year - 2009
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.2008.00498.x
Subject(s) - chemistry , cysteine , context (archaeology) , membrane , membrane protein , chemical shift , nuclear magnetic resonance spectroscopy , proton nmr , crystallography , stereochemistry , biochemistry , enzyme , biology , paleontology
Abstract Proteorhodopsin (PR) is a light‐driven proton pump found in near‐surface marine γ‐proteobacteria. The green absorbing variant has three cysteines at positions 107, 156 and 175. We probed the accessibility of these residues by 19 F‐MAS NMR. For this purpose, an efficient but simple protocol for chemical fluorine labeling of accessible cysteines in membrane proteins was established. This one‐step reaction was applied to detergent‐solubilized PR before reconstitution into phospholipids. All three cysteines could be labeled and showed distinct 19 F chemical shifts with different integral intensities. The accessibility of these cysteines is discussed in the context of a homology model. With the chemical cysteine labeling procedure shown here, an attractive option for site‐directed solid‐state NMR studies on other membrane proteins is offered due to the high intrinsic sensitivity of 19 F‐MAS NMR.