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Influence of pH upon Surface‐enhanced Enzyme‐catalyzed Luminol Chemiluminescence at Vicinity of Nanoscale‐corrugated Gold and Silver Films
Author(s) -
Ou Meigui,
Lu Guowei,
Shen Hong,
Descamps Armel,
Marquette Christophe André,
Blum Loïc Jacques,
Roux Stéphane,
Tillement Olivier,
Cheng Bolin,
Perriat Pascal
Publication year - 2008
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.2008.00350.x
Subject(s) - luminol , chemiluminescence , catalysis , peroxidase , chemistry , adsorption , photochemistry , nanoscopic scale , biochip , inorganic chemistry , metal , nuclear chemistry , materials science , enzyme , nanotechnology , organic chemistry
Au and Ag biochips were fabricated to investigate the influence of pH upon the chemiluminescence (CL) of luminol at vicinity of surface‐adsorbed peroxidase. A nanoscaled‐corrugation of the metal induces an enhancement of the luminol CL which is maximal in the pH range favoring peroxidase catalysis and greater for gold than for silver. This is the proof that, in the CL process, the reactions involving peroxidase are surface‐enhanced near corrugated surfaces.