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Ultraviolet Resonance Raman Microprobe Spectroscopy of Photosystem II
Author(s) -
Chen Jun,
Barry Bridgette A.
Publication year - 2008
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.2008.00298.x
Subject(s) - resonance raman spectroscopy , photochemistry , raman spectroscopy , chemistry , photosynthesis , photosystem i , photosystem ii , electron transfer , p700 , microprobe , spectroscopy , resonance (particle physics) , oxygen , ultraviolet , materials science , biochemistry , optoelectronics , optics , atomic physics , physics , organic chemistry , mineralogy , quantum mechanics
Photosystem II (PSII) carries out photosynthetic oxygen production and is responsible for the maintenance of aerobic, heterotrophic life. In PSII, protein amino acid residues play an important role in the light‐driven electron transfer reactions. Here, we describe an approach to enhancing vibrational signals from PSII proteins through ultraviolet resonance Raman (UVRR) and a microprobe jet flow technique. Our work shows that pump‐probe UVRR can be used to monitor intermediates during photosynthetic oxygen evolution.