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Photodegradation of Soil Organic Matter and its Effect on Gram‐negative Bacterial Growth
Author(s) -
Bosio Gabriela N.,
Gara Pedro David,
Einschlag Fernando S. García,
Gonzalez Mónica C.,
Del Panno María Teresa,
Mártire Daniel O.
Publication year - 2008
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.2007.00274.x
Subject(s) - photodegradation , chemistry , fourier transform infrared spectroscopy , bioavailability , photochemistry , humic acid , substrate (aquarium) , bacteria , photodissociation , bacterial growth , absorption (acoustics) , organic matter , nuclear chemistry , environmental chemistry , organic chemistry , catalysis , materials science , chemical engineering , biology , photocatalysis , ecology , bioinformatics , fertilizer , genetics , engineering , composite material
To learn more about the role of the reactive oxygen species (ROS) in the production of bioavailable products of the dissolved organic matter, we investigate here the effect of the photolysis (λ exc > 320 nm) of a soil extract (SE) on the growth of bacteria isolated from the same soil as used for obtaining the extract. Comparative experiments with Aldrich humic acid (AHA) as substrate were performed. The photodegradation of the SE was evaluated with different techniques—UV–visible absorption spectroscopy, fluorescence excitation emission matrices (EEM) and Fourier transform infrared spectroscopy (FTIR). Known ROS scavengers were employed to study the effect of photochemically produced ROS on the photodegradation of the substrates. To evaluate the effect of irradiation on the bioavailability of the SE and AHA, photolyzed and nonphotolyzed substrates were added to different culture media and the growth of Pseudomonas sp. isolated from the soil and a strain of Escherichia coli were studied. The different results obtained were assigned to the dissimilar metabolisms of both bacteria.