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Effect of X‐Radiation on Lipid Peroxidation and Antioxidant Systems in Rats Treated with Saponin‐containing Compounds
Author(s) -
Yalinkilic Omer,
Enginar Huseyin
Publication year - 2007
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.2007.00240.x
Subject(s) - chemistry , lipid peroxidation , malondialdehyde , antioxidant , retinol , glutathione , vitamin , vitamin e , vitamin c , food science , carotene , medicine , endocrinology , biochemistry , enzyme
The aim of this study was to investigate the effect of three saponin‐containing plant species extracts ( Aesculuc hippocastanum L. seed extract [AHE], Medicago sativa L . extract [MSE] and Spinacia oleracea L. extract [SOE]) on lipid peroxidation and on antioxidant systems in rats exposed to X‐rays (XR). The rats were divided into three categories. The first category served as controls and received only a standard diet. The second category served as the radiation group and received 5 and 10 Gy XR dose. The third category (XR+extract‐treated) received plant extracts (25.0 or 50.0 mg kg −1 live weight) and 5 or 10 Gy XR dose. Blood samples were analyzed for their content of malondialdehyde (MDA), reduced glutathione (GSH), plasma vitamin C, β‐carotene and retinol. In animals receiving XR, the plasma MDA ( P < 0.001) value significantly increased but the level of GSH ( P < 0.01), vitamin C ( P < 0.001), retinol and β‐carotene ( P < 0.001) decreased significantly with increasing XR doses. In the XR+extract‐treated groups, the concentrations of MDA increased significantly with increasing radiation but their concentrations decreased significantly with increasing extract concentrations. Plasma concentrations of GSH, β‐carotene, retinol and vitamin C in XR+extract‐treated groups decreased significantly with increasing XR dose but their concentrations increased with increasing extract doses. Further, comparison of blood samples of XR+extract‐treated groups with those from the control group showed that GSH, β‐carotene, retinol and vitamin C values increased significantly but that MDA values decreased significantly. The results showed that all extracts have enhanced the antioxidant status and decreased the incidence of free radical‐induced lipid peroxidation in blood samples of rats exposed to XR. However, the antioxidant effect of AHE‐administered animals was more effective than that of MSE‐ and SOE‐administered whole‐body XR rats. We conclude that the supplementation with saponin‐containing extracts may serve to reinforce the antioxidant systems, thus having protective effect against cell damage by XR.