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Ultraviolet‐A and ‐B Differentially Modify the Tyrosine‐Kinase Profile of Human Keratinocytes and Induce the Expression of Arg †
Author(s) -
Klosner Gabriele,
Varecka Roland,
Knobler Robert,
Trautinger Franz
Publication year - 2007
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.2007.00235.x
Subject(s) - downregulation and upregulation , microbiology and biotechnology , tyrosine kinase , messenger rna , carcinogenesis , gene expression , tyrosine , keratinocyte , biology , signal transduction , chemistry , gene , cell culture , biochemistry , genetics
To investigate the expression profile of protein tyrosine kinases (PTKs) in normal human epidermal keratinocytes (NHEK) in response to UVA and UVB we employed a reversed transcriptase polymerase chain reaction (PCR) approach using degenerate primers derived from the conserved catalytic domain of PTKs. Quantitative real‐time PCR with specific primers was used to confirm the influence of UV on the expression of the identified PTKs. Arg (Abelson‐related gene, Abl2) was the PTK with the highest prevalence (30% of all PTKs) and UVA led to a further induction of Arg expression reaching nine‐fold mRNA baseline expression at 17 h after irradiation. UVB was followed by an initial downregulation and a subsequent increase in Arg mRNA reaching five‐fold baseline levels after 24 h. We conclude that UVA and UVB differentially modify the expression of PTKs in NHEK, and that Arg appears to have a major role in the response of keratinocytes to UV. These results provide a basis for further studies of PTK in UV‐induced signaling that regulates protective responses, cell growth and carcinogenesis in the skin.