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Photodynamic Action of Benzo[a]pyrene in K562 Cells
Author(s) -
De Moraes Vaz Batista Filgueira Daza,
Paula Salomão de Freitas Diana,
Paula de Souza Votto Ana,
Fillmann Gilberto,
Maria Monserrat José,
Alicia Geracitano Laura,
Santos Trindade Gilma
Publication year - 2007
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.2007.00169.x
Subject(s) - chemistry , phototoxicity , benzo(a)pyrene , reactive oxygen species , photosensitizer , viability assay , pyrene , singlet oxygen , lipid peroxidation , dna damage , oxidative stress , carcinogen , intracellular , microbiology and biotechnology , biochemistry , dna , biophysics , apoptosis , photochemistry , in vitro , oxygen , biology , organic chemistry
Benzo[a]pyrene (BaP) is ubiquitously distributed in the environment, being considered the most phototoxic element among polycyclic aromatic hydrocarbon (PAHs). In presence of oxygen, PAHs can act as a photosensitizer by means of promoting photo‐oxidation of biological molecules (photodynamic action, PDA). Thus, the present study analyzed the photodynamic action of BaP under UVA irradiation (BaP + UVA) and its oxidative effects on K562 cells. The evaluation of BaP kinetics showed that the highest intracellular concentration occurred after 18 h of incubation. Cell viability, reactive oxygen species (ROS) generation, lipid peroxidation, DNA damage (breaks and DNA–protein cross‐link [DNAPC]) were assessed after exposure to BaP, UVA and BaP plus UVA irradiation (BaP + UVA). Cell viability was decreased just after exposure to BaP + UVA. Lipid peroxidation and DNA breaks increased after BaP + UVA exposure, while the DNAPC increased after BaP, UVA and BaP + UVA exposure, suggesting that BaP + UVA effects were a consequence of both type II (producing mainly singlet oxygen) and type I (producing others ROS) mechanisms of PDA.