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New Insights into Lung Surfactant Monolayers Using Vibrational Sum Frequency Generation Spectroscopy
Author(s) -
Ma Gang,
Allen Heather C.
Publication year - 2006
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.2006.tb09807.x
Subject(s) - monolayer , dipalmitoylphosphatidylcholine , pulmonary surfactant , tripalmitin , chemistry , chromatography , analytical chemistry (journal) , langmuir , chemical engineering , crystallography , phospholipid , organic chemistry , membrane , adsorption , biochemistry , phosphatidylcholine , engineering
At the air‐water interface, interfacial molecular structure, intermolecular interactions, film relaxation and film respreading of model lung surfactant monolayers were studied using vibrational sum frequency generation (VSFG) spectroscopy combined with a Langmuir film balance. Chain‐perdeuterated dipalmitoylphosphatidylcholine (DPPC‐ d62 ), palmitoyloleoylphosphatidylglycerol (POPG), palmitic acid (PA) and tripalmitin were investigated. In the DPPC‐ d62 ‐PA binary monolayer, PA showed a condensing effect on the DPPC chains. On the contrary, in the DPPC‐ d62 ‐POPG binary monolayer, POPG showed a fluidizing effect on the DPPC chains. In the ternary monolayer system of DPPC‐ d62 ‐POPG–PA, the balance between the fluidizing and the condensing effect was also observed. In addition, the film relaxation behavior of DPPC‐ d62 and the enhanced film stability of DPPC‐ d62 caused by the addition of tripalmitin were observed. Real‐time VSFG was also employed to study the respreading properties of a complex lung surfactant mixture containing DPPC‐ d62 , POPG, PA and KL 4 (a mimic of SP‐B) peptide, which revealed DPPC enrichment after film comoression.