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Kinetics of Protoporphyrin IX Formation in Rat Oral Mucosa and Skin After Application of 5‐Aminolevulinic Acid and its Methylester ¶
Author(s) -
Kristiansson Stefan,
Juzeniene Asta,
Juzenas Petras,
Iani Vladimir,
Löfgren Lennart,
Moan Johan
Publication year - 2005
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.2005.tb00199.x
Subject(s) - protoporphyrin ix , oral mucosa , chemistry , kinetics , fluorescence , pharmacology , drug , protoporphyrin , biochemistry , photodynamic therapy , pathology , medicine , physics , porphyrin , organic chemistry , quantum mechanics
The kinetics of accumulation of protoporphyrin IX (PpIX) after topical application of 5‐aminolevulinic acid (ALA) and its methylester (5‐aminolevulinic acid methylester [ALA‐Me]) was studied on rat oral mucosa. The accumulation of PpIX in mucosa and skin after intravenous injection of ALA and ALA‐Me was also studied. The elimination rate of PpIX was dependent on drug and dose as well as on administration route. Application of ALA on rat oral mucosa and skin caused a systemic effect with PpIX building up in remote skin sites not exposed to the drugs. No such systemic effect was seen after application of ALA‐Me either in mucosa or on skin. Intravenous injection of the drugs (0.2 g/kg) leads to more fluorescence in the skin than topical application of the drug (20%). For mucosa, the opposite is true. Maximal PpIX fluorescence appeared later after application of high concentrations of the drugs (around 8 h for 5% and 20% wt/wt) than after application of low concentrations (around 3–5 h for 1% and 2% wt/wt).