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Two‐photon Excitation Induced Fluorescence of a Trifluorophore‐labeled DNA ¶
Author(s) -
Jockusch Steffen,
Li Zengmin,
Ju Jingyue,
Turro Nicholas J.
Publication year - 2005
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.2005.tb00180.x
Subject(s) - fluorescence , excitation , two photon excitation microscopy , chemistry , laser , fluorescence in the life sciences , laser induced fluorescence , photochemistry , analytical chemistry (journal) , atomic physics , physics , optics , chromatography , quantum mechanics
Two‐photon excitation of a trifluorophore (6‐carboxyfluorescein, N, N, N′, N′ ‐tetramethyl‐6‐carboxyrhodamine and cyamine‐5 monofunctional dye) labeled DNA, which has a scaffold of 26 nucleotides, was achieved using focused laser light of a Q‐switched Nd‐YAG laser (1064 nm). The observed fluorescence signature (emission ratio from the three fluorophores) of the labeled DNA after two‐photon excitation is very different from the fluorescence signatures produced by one‐photon excitation at different wavelenght. The additional fluorescence signatures produced by two‐photon excitation of the fluorescent oligonucleotides will facilitate their use as combinatorial fluorescence energy transfer tags for multiplex genetic analysis.