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Redox Photochemistry of Thiouredopyrenetrisulfonate ¶
Author(s) -
Kottyar Alexander B.,
Borovok Natalia,
Khoroshyy Petro,
Tenger Katalin,
Zimányi László
Publication year - 2004
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.2004.tb01264.x
Subject(s) - redox , photochemistry , chemistry , electron transfer , excited state , covalent bond , photoinduced electron transfer , triplet state , electron exchange , molecule , macromolecule , reaction rate constant , kinetics , inorganic chemistry , organic chemistry , ion , biochemistry , physics , quantum mechanics , nuclear physics
ABSTRACT 1‐Thiouredopyrene‐3,6,8‐trisulfonate (TUPS) has recently been used as a photoinduced covalent redox label capable of reducing various cofactors of proteins. A new reaction of this dye, whereby its excited triplet state oxidizes suitable electron donors, is now reported. The characteristic difference spectrum of the reduced radical of TUPS is determined. We also observe the self‐exchange electron transfer between two TUPS molecules in their triplet excited states and determine the reaction scheme and the rate constants of the various pathways in the process of triplet depletion. The ability of photoexcited TUPS to withdraw an electron from reduced cytochrome‐c is also observed. It is thus demonstrated that TUPS is an appropriate photoinduced covalent redox label for initiating both the oxidative and reductive phases of electron transfer processes in biological macromolecules.