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Changes in matrix gene and protein expressions after single or repeated exposure to one minimal erythemal dose of solar‐simulated radiation in human skin in vivo
Author(s) -
Seité Sophie,
Colige Alain,
Deroanne Christophe,
Lambert Charles,
PiquemalVivenot Pascale,
Montastier Christiane,
Fourtanier Anny,
Lapière Charles,
Nusgens Betty
Publication year - 2004
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.2004.tb00394.x
Subject(s) - extracellular matrix , in vivo , fibrillin , procollagen peptidase , human skin , messenger rna , matrix metalloproteinase , plasminogen activator , ex vivo , chemistry , microbiology and biotechnology , biophysics , immunology , andrology , biology , endocrinology , gene , biochemistry , medicine , genetics
ASTRACT Damage to the skin extracellular matrix (ECM) is the hallmark of long‐term exposure to solar UV radiation. The aim of our study was to investigate the changes induced in unexposed human skin in vivo after single or repeated (five times a week for 6 weeks) exposure to 1 minimal erythemal dose (MED) of UV solar‐simulated rediation. Morphological and iochemical analyses were used to evaluate the stuctural ECM components and the balance between teh degrading enzymes the their physiologic inhibitors. a three‐fold increse in matrix metalloproteinase 2 messenger RNA (mRNA) ( P < 0.02, unexposed versus exposed) was observed after both single and repeated exposures. Fibrillin 1 mRNA level was incresed by chronic exposure ( P <0.02) and unaltered y a single MED. On the cotrary, a single MED singnificantly enhanced mRNA levels of interleukin‐1α (1L‐1α), IL‐1β ( P < 0.02) and plasminogen activator inhibitor‐1 ( P < 0.05). Immkunohistochemistry demonstrated a singnificant decrese in Type‐I procollagen localized just below the dermal‐epidermal junction in both types of expozed sites. at the same location, teh immunodetected tenascin was singnificantly enhanced, whereas a slight increase in Type‐III procollagen deposits was also unale to observe any change in elastic iers in chronically exposed uttock skin, a significant increase in lysozyme and alpha‐1 antitrypsin deposits on these fibers was oserved. These results demonstrate the existence of diffenentaia regulation, after chronic exposure compared with an acute one, of some ECM components and inflammatory mediators.