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Photo‐CIDNP Study of the Interaction of Tyrosine with Nifedipine. An Attempt to Model the Binding Between Calcium Receptor and Calcium Antagonist Nifedipine ¶
Author(s) -
Polyakov Nikolal E.,
Taraban Marc B.,
Leshina Tatyana V.
Publication year - 2004
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.2004.tb00130.x
Subject(s) - cidnp , chemistry , nifedipine , binding site , antagonist , calcium , receptor , tyrosine , biophysics , stereochemistry , biochemistry , radical , biology , organic chemistry
This article proposes a new approach to the modeling of the molecular‐level mechanism of ligand‐receptor interaction for Ca 2+ receptor binding site. Chemically induced dynamic nuclear polarization (CIDNP) technique has been used to unravel fine details of the reaction in the model system composed of one of the known Ca 2+ antagonist drugs, nifedipine (NF), and isolated amino acid residuals ( e.g. tyrosine [Tyr]) of Ca 2+ receptor binding site. It has been conclusively demonstrated that the reaction between NF and Tyr resulting in the oxidation product—nitroso form of NF—obeys the radical mechanism. CIDNP data in combination with the results of mathematical modeling of the structures of ligandreceptor complexes have allowed to propose the mechanism of the interaction of NF with Ca 2+ receptor binding site.