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Surface Plasmon‐coupled Polarized Emission of N‐Acetyl‐ l ‐Trytophanamide ¶
Author(s) -
Gryczynski Ignacy,
Malicka Joanna,
Lukomska Joanna,
Gryczynski Zygmunt,
R. Lakowicz Joseph
Publication year - 2004
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.2004.tb00118.x
Subject(s) - surface plasmon resonance , surface plasmon , prism , analytical chemistry (journal) , materials science , layer (electronics) , chemistry , ultraviolet , fluorophore , plasmon , optics , fluorescence , optoelectronics , nanoparticle , nanotechnology , physics , chromatography
We report an observation of ultraviolet (UV) surface plasmon‐coupled emission (SPCE) of N‐acetyl‐ l ‐tryptophanamide (NATA). The sample was spin coated from poly(vinyl alcohol) (PVA) solution on 20 nmaluminium film deposited on a quartz substracte. The directional UV SPCE occurs within a well‐defined narrow angle at 52dG from the normal to the coupling hemicylinder quartz prism. The NATA directional emission is highly p polarized as expected for surface plasmon‐coupled radiation.The 10 nm protective SiO 2 layer deposited on top of the aluminum film significantly neutralized the fluorophore quenching by the metal surface. SPCE of NATA demonstratees a remarkable intrinsic dispersive property–the maximum of the emission spectrum depends on the observation angle. The efficient spectral resolution of SPCE can be used in the construction of miniaturized spectrofluorometers. The observation of SPCE of tryptophan opens a new possibility for the study of many unlabeled proteins with the technique complementary to surface plasmon resonance analysis.