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Binding of Metal Ions to Melanin and Their Effects on the Aerobic Reactivity ¶
Author(s) -
Hong Lian,
Liu Yan,
Simon John D.
Publication year - 2004
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.2004.tb00117.x
Subject(s) - chemistry , reactivity (psychology) , metal , melanin , sepia , amine gas treating , metal ions in aqueous solution , hydroxyl radical , reactive oxygen species , oxygen , inorganic chemistry , ion , nuclear chemistry , mass spectrometry , radical , organic chemistry , biochemistry , chromatography , linguistics , medicine , alternative medicine , philosophy , pathology , officinalis
The binding of Mg(II), Ca(II), Zn(II), Cu(II) and Fe(III) to ETDA‐washed Sepia melanin is quantified by inductively coupled plasma mass spectrometry. By monitoring the solution pH change associated with metal uptake, it is concluded that Mg(II), Ca(II) and Zn(II) bind to carboxylic acid groups in melanin, Cu(II) binds to hydroxyl (OH) groups and Fe(III) binds to OH or amine groups. The aerobic reactivity of melanins with different metal contents is analyzed by examining their ability to cause strand breaks in super‐coiled pUC18 DNA. Cu(II)‐ and Fe(III)‐enriched melanins induce the most damage. Hydroxyl radical, *OH, is proposed to be one of the reactive oxygen species responsible.