Premium
Phototoxic Effect of Visible Light on Porphyromonas gingivalis and Fusobacterium nucleatum: An In Vitro Study ¶ †
Author(s) -
Feuerstein Osnat,
Persman Nir,
Weiss Ervin I.
Publication year - 2004
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.2004.tb00106.x
Subject(s) - fusobacterium nucleatum , porphyromonas gingivalis , phototoxicity , enterococcus faecalis , chemistry , visible spectrum , irradiation , streptococcus mutans , microbiology and biotechnology , materials science , nuclear chemistry , bacteria , in vitro , optoelectronics , medicine , periodontitis , dentistry , biology , escherichia coli , biochemistry , physics , genetics , nuclear physics , gene
The antibacterial effect of visible light irradiation combined with photosensitizers has been reported. The objective of this was to test the effect of visible light irradiation without photosensitizers on the viability of oral microorganisms. Strains of Porphyromonas gingivalis, Fusobacteriurm nucleatum, Streptococcus mutans and Streptococcus faecalis in suspension or grown on agar were exposed to visible light at wavelengths of 400–500 nm. These wavelengths are used to photopolymerize composite resins widely used for dental restoration. Three photocuring light sources, quartz‐tungsten‐halogen lamp, light‐emitting diode and plasma‐arc, at power densities between 260 and 1300 mW/cm 2 were used for up to 3 min. Bacterial samples were also exposed to a near‐infrared diode laser (wavelength, 830 nm), using identical irradiation parameters for comparison. The results show that blue light sources exert a phototoxic effect on P. gingivalis and F. nucleatum . The minimal inhibitory dose for P. gingivalis and F. nucleatum was 16–62 J/cm 2 , a value significantly lower than that for S. mutans and S. faecalis (159–212 J/cm 2 ). Near‐infrared diode laser irradiation did not affect any of the bacteria tested. Our results suggest that visible light sources without exogenous photosensitizers have a phototoxic effect mainly on Gram‐negative periodontal pathogens.