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Rapid Communication: Direct Optical Detection of Singlet Oxygen from a Single Cell ¶
Author(s) -
Zebger Ingo,
Snyder John W.,
Andersen Lars Klembt,
Poulsen Lars,
Gao Zhan,
Lambert John D. C.,
Kristiansen Uffe,
Ogilby Peter R.
Publication year - 2004
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.2004.tb00015.x
Subject(s) - singlet oxygen , photosensitizer , phosphorescence , oxygen , photochemistry , irradiation , chemistry , singlet state , single cell analysis , materials science , cell , fluorescence , optics , physics , atomic physics , excited state , biochemistry , organic chemistry , nuclear physics
Singlet oxygen has been detected in single nerve cells by its weak 1270 nm phosphorescence (a 1 Δ g →X 3 Σ g − ) upon irradiation of a photosensitizer incorporated in the cell. Thus, one can now consider the application of direct optical imaging techniques to mechanistic studies of singlet oxygen at the single‐cell level.