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Significance of Dosimetry in Photodynamic Therapy of Injured Arteries: Classification of Biological Responses
Author(s) -
Adili Farzin,
Eps Randolph G. Statius,
LaMuraglia Glenn M.
Publication year - 1999
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1999.tb08267.x
Subject(s) - photodynamic therapy , medicine , dosimetry , artery , thrombus , microgram , occlusion , intimal hyperplasia , nuclear medicine , radiology , urology , chemistry , in vitro , biochemistry , organic chemistry , smooth muscle
With conflicting results in the literature on the ability of photodynamic therapy (PDT) to inhibit intimal hyperplasia (IH), the present study systematically investigated the effects of drug and light dosimetry on the biologic responses in the artery wall. The rat common carotid artery was balloon‐injured and pressurized with benzopor‐phyrin‐derivative monoacid ring (BPD). Then, PDT was performed with an external laser at different fluences and the biologic responses of the artery wall were histologically examined at 24 h and at 2 weeks. Photodynamic therapy effects on injured arteries can be classified into four stages: low‐dose PDT using 0.5 (ig/mL BPD at 50 J/cm 2 (stage I) resulted in incomplete cell eradication and significant IH at 2 weeks. Irradiation with 100 J/cm 2 at the same BPD concentration (stage II) completely eradicated the cells in the artery wall at 24 h but still led to IH at 2 weeks. However, 25 (tg/mL BPD at 100 J/cm 2 (stage in) resulted in total cell eradication at 24 h and inhibition of IH at 2 weeks. In contrast, high‐dose PDT with 25 μg/mL BPD and 200 J/cm 2 (stage TV) led to thrombus development and vascular occlusion at 24 h. These data, demonstrating the different stages of PDT effects on injured arteries, emphasize the critical importance of appropriate PDT dosimetry for the effective inhibition of IH.