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Apoptosis Induction in Nonirradiated Human HL‐60 and Murine NSO/2 Tumor Cells by Photoproducts of lndole‐3‐acetic Acid and Riboflavin
Author(s) -
Edwards A. M.,
Barredo F.,
Silva E.,
Ioannes A. E.,
Becker M. I.
Publication year - 1999
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1999.tb08264.x
Subject(s) - apoptosis , flow cytometry , chemistry , dna fragmentation , riboflavin , acetic acid , fragmentation (computing) , tryptophan , programmed cell death , microbiology and biotechnology , dna , cell , cell culture , biochemistry , biology , amino acid , genetics , ecology
The effect of the photoproducts of indole‐3‐acetic acid sensitized by riboflavin on nonirradiated human HL‐60 and murine NSO/2 tumor cells was studied. Severe damage with a dose‐response effect was observed on both cell types. The effect was greater than that previously described for the tryptophan riboflavin photoproducts. Electron microscopy studies and flow cytometry analysis of DNA fragmentation allowed us to conclude that the photoproducts studied in this work induce cell death by an apoptotic mechanism.