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Anion‐lnduced Fluorescence Quenching of a New Zwitterionic Biacridine Derivative
Author(s) -
Werner Tobias,
Fahnrich Karsten,
Huber Christian,
Wolfbeis Otto S.
Publication year - 1999
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1999.tb08255.x
Subject(s) - chemistry , bromide , fluorescence , iodide , hepes , quenching (fluorescence) , chloride , halide , ion , derivative (finance) , analytical chemistry (journal) , inorganic chemistry , photochemistry , chromatography , organic chemistry , biochemistry , physics , quantum mechanics , financial economics , economics
The effect of halides and different buffer anions on the quenching of the fluorescence of the new probe 10,10′‐bis(3‐sulfopropyl)‐9,9′‐biacridine (SPBA) has been studied using fluorescence and decay time measurements. The linearity of the Stern‐Volmer plot indicates that fluorescence quenching by halides can be described reasonably well by a single‐exponential decay with a K of 4.06 times 10 6 M ‐1 s ‐1 for chloride, 7.83 times 10 6 M ‐1 s ‐1 for bromide and 1.12 times 10 7 M ‐1 s ‐1 for iodide. We have found that SPBA is collisionally quenched also by the buffers 3‐( N ‐mor‐pholino)propanesulfonic acid (MOPS) and N ‐2‐hydroxy‐ethylpiperazine‐ N ′‐ethansulfonic acid (HEPES). The bi‐molecular rate constants are 1.67 × 10 6 M ‐l s ‐1 for HEPES and 1.44 times 10 6 M ‐1 s ‐1 for MOPS.