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Femtosecond Two‐photon Excited Fluorescence of Melanin*
Author(s) -
Teuchner Klaus,
Freyer Wolfgang,
Leupold Dieter,
Volkmer Andreas,
Birch David J. S.,
Altmeyer Peter,
Stucker Markus,
Hoffmann Klaus
Publication year - 1999
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1999.tb07982.x
Subject(s) - fluorescence , excited state , two photon excitation microscopy , femtosecond , absorption (acoustics) , photochemistry , chemistry , materials science , optics , atomic physics , physics , laser
. Fluorescence of synthetic melanin in dimethyl sulfoxide has been excited by two‐photon absorption at 800 run, using 120 fs pulses with photon flux densities 10 27 cm 2 s 1 . The shortest main component of the three‐exponential decay of fluorescence is 200 ± 2 ps. The overall spectral shape is red‐shifted with respect to the 400 nm excited fluorescence. Two‐photon excited melanin fluorescence also has been measured from excised samples of healthy human skin tissue. Because of the selectivity of melanin excitation via resonant two‐photon absorption, it is hypothesized that fluorescence excited in this way may yield information on malignant transformation.