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Photoproduct Formation from a Zinc Benzochlorin Iminium Salt Detected by Fluorescence Microscopy
Author(s) -
Amit Ifat,
Malik Zwi,
Kessel David
Publication year - 1999
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1999.tb03349.x
Subject(s) - iminium , zinc , salt (chemistry) , fluorescence , fluorescence microscope , chemistry , microscopy , photochemistry , optics , biochemistry , physics , organic chemistry , catalysis
— When examined by fluorescence microscopy, tumor cells loaded with a zinc benzochlorin iminium salt showed a very faint deep‐red fluorescence that was rapidly transformed to a substantially brighter red‐orange fluorescence. Fourier transfer spectroscopy analysis with a red‐sensitive detection system revealed that an initial fluorescence at 770 nm was gradually converted to 640 nm fluorescence during excitation. Image analysis showed that photoproduct formation was accompanied by a change in the site of drug localization from the cytoplasm to the nucleus. These studies illustrate the power of interferometry for the characterization of photoproducts and changes in sensitizer localization during photoproduct formation.