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Photoregulation of DMA Photolyases in Broom Sorghum Seedlings
Author(s) -
Hada Megumi,
Buchholz Günther,
Hashimoto Tohru,
Nikaido Osamu,
Wellmann Eckard
Publication year - 1999
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1999.tb03346.x
Subject(s) - photolyase , pyrimidine dimer , sorghum , dna , blue light , chemistry , enzyme , biology , dna repair , biochemistry , materials science , optoelectronics , ecology
— Using dark‐grown 3 day‐old Sorghum bicolor seedlings, photorepair of cyclobutane pyrimidine dimers (CPD) by CPD‐photoIyase and pyrimidine‐(6–4)pyrimidinone photoproducts ([6–4]PP) by [6–4]PP‐photolyase was studied in vivo and in vitro. The two types of DNA photoproducts were measured with specific monoclonal antibodies. Sorghum seedlings have different photorepair enzymes for CPD and [6–4]PP. The CPD‐photolyase was found to be increased in response to light with maximal efficiency in the UVA/blue spectral range, whereas [6–4]PP‐photolyase was not influenced by light.